Tbx5 is required for forelimb bud formation and continued outgrowth
نویسندگان
چکیده
منابع مشابه
Tbx5 is dispensable for forelimb outgrowth.
Tbx5 is essential for initiation of the forelimb, and its deletion in mice results in the failure of forelimb formation. Misexpression of dominant-negative forms of Tbx5 results in limb truncations, suggesting Tbx5 is also required for forelimb outgrowth. Here we show that Tbx5 is expressed throughout the limb mesenchyme in progenitors of cartilage, tendon and muscle. Using a tamoxifeninducible...
متن کاملTbx5 Buffers Inherent Left/Right Asymmetry Ensuring Symmetric Forelimb Formation
The forelimbs and hindlimbs of vertebrates are bilaterally symmetric. The mechanisms that ensure symmetric limb formation are unknown but they can be disrupted in disease. In Holt-Oram Syndrome (HOS), caused by mutations in TBX5, affected individuals have left-biased upper/forelimb defects. We demonstrate a role for the transcription factor Tbx5 in ensuring the symmetric formation of the left a...
متن کاملRetinoic acid is required for the initiation of outgrowth in the chick limb bud
BACKGROUND Retinoic acid (RA) is present in the chick limb bud, and excess RA induces limb duplications. Here, we have investigated the role of endogenous RA during chick limb development by preventing the synthesis of RA and testing the effect on various genes expressed during limb initiation and outgrowth. RESULTS We demonstrate that the stage 20/21 limb bud synthesizes didehydroretinoic ac...
متن کاملTbx4 is not required for hindlimb identity or post-bud hindlimb outgrowth.
Tbx4 is a crucial gene in the initiation of hindlimb development and has been reported as a determinant of hindlimb identity and a presumptive direct regulator of Fgf10 in the limb. Using a conditional allele of Tbx4, we have ablated Tbx4 function before and after limb initiation. Ablation of Tbx4 before expression in the hindlimb field confirms its requirement for limb bud outgrowth. However, ...
متن کاملTbx5 is required for avian and Mammalian epicardial formation and coronary vasculogenesis.
RATIONALE Holt-Oram syndrome is an autosomal dominant heart-hand syndrome caused by mutations in the TBX5 gene. Overexpression of Tbx5 in the chick proepicardial organ impaired coronary blood vessel formation. However, the potential activity of Tbx5 in the epicardium itself, and the role of Tbx5 in mammalian coronary vasculogenesis, remains largely unknown. OBJECTIVE To evaluate the consequen...
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ژورنال
عنوان ژورنال: Development
سال: 2003
ISSN: 1477-9129,0950-1991
DOI: 10.1242/dev.00473